Isolation and identification of embryonic pancreatic tissues in mice
10.3760/cma.j.issn.1674-6090.2012.06.002
- VernacularTitle:小鼠胚胎胰腺组织分离及内分泌功能鉴定
- Author:
Jun YANG
;
Guangwen ZHOU
- Publication Type:Journal Article
- Keywords:
Embryo;
Organ primordia;
Isolation;
Immunohistochemistry;
Immunofluorescence;
Mice
- From:
Journal of Endocrine Surgery
2012;6(6):367-370
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the isolation technique of embryonic pancreatic tissues in mice and to identify the isolated embryonic pancreatic tissue by morphology and endocrine function test.Methods Pancreatic tissues from embryonic(E) 11.5-16.5 d C57BL/6 mouse embryos were surgically isolated under the stereomicroscope.E12.5 d,E14.5 d,E16.5 d embryonic pancreas tissues were identified by conventional HE staining,immunohistochemistry and immunofluorescence.The endocrine functions of embryonic pancreatic tissues were measured by glucose stimulation test.Results Embryonic pancreatic tissues of mice over embryonic 12.5days can be easily isolated.Embryonic pancreatic tissues of mice younger than embryonic 12.5 days were hard to achieve and these pancreatic tissues could only be isolated according to the relationship with the adjacent organs.Histological and ELISA examinations showed that early embryonic pancreatic tissues could express and secrete insulin and the insulin level was gradually increased with developmental time.Conclusion A good command of the knowledge of the development and anatomy of mouse embryo help improve isolation of embryonic pancreas,which provides therapeutic potential for embryonic pancreatic tissue transplantation.
