Construction of human Egr-1 promoter and its response to ionizing radiation in tumor cells
10.3321/j.issn:1007-8738.2009.11.003
- VernacularTitle:人Egr-1启动子的克隆及其在肿瘤细胞中的辐射诱导反应
- Author:
Xiaojie XU
;
Lihua DING
;
Lingxue WANG
;
Xi QIN
;
Long CHENG
;
Kai JIANG
;
Qinong YE
- Publication Type:Journal Article
- Keywords:
Egr-1 promoter;
ionizing radiation;
promoter activity
- From:
Journal of Cellular and Molecular Immunology
2009;25(11):973-975
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To construct human Egr-1 promoter luciferase reporter system and study its activity induced by i-onizing radiation. METHODS: Egr-1 promoter was obtained by human genomic PCR and cloned into pGL3-basic vector. After transfection of recombinant plasmid into human tumor cells, the Egr-1 promoter activity induced by ionizing radiation was detected by luciferase reporter assay. RESULTS: The luciferasy reporter system of Egr-1 promoter was successfully constructed. The activity of Egr-1 promoter was substantially increased after different doses of IR and reached to the peak at the time point of 48h after IR. CONCLUSION: The Egr-1 promoter was constructed in this study showed IR inducible activity in tumor cells, laying foundation for the research of radiation, mediated gene therapy.
