Impact of MafA gene on blood sugar level in diabetic rats
10.3760/cma.j.issn.1674-6090.2011.01.010
- VernacularTitle:MafA基因对糖尿病大鼠血糖影响的研究
- Author:
Daorong WANG
;
Yong ZHAO
;
Liuhua WANG
;
Yongkun LI
- Publication Type:Journal Article
- Keywords:
MafA gene;
Diabetes mellitus;
Insulin;
Blood glucose
- From:
Journal of Endocrine Surgery
2011;05(1):30-33
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of the MafA therapy for blood slucose control in diabetic rats.Methods Rats were divided into 3 groups:treatment group,DM group and control group.In DM group,Wistar rats were rendered diabetic by intrvenous injection of streptozotocin(STZ).In treatment group,mixture of MafA and liposomes at the volume ratio of 1:1 was injected to the portal vein of the diabetic rats.Blood glucose change in the diabetic rats Was measured.Results ① For treatment group,blood slucose decreased from 20.6 mmol/L-22.8 mmol／L to 13.6 mmol／L-14.8 mmol／L and plasma insulin level was significantly elevated for a duration about 2 weeks.②After treatment,blood slucose level significantly decreased compared to DM group (P<0.05).③ Plasma insulin level Was significantly higher than that in DM group(P<0.05).④ Expression of MafA mRNA can be detected in the liver oftreatment group while it Was not found in DM group.Immunohistochemical analysis revealed that insulin expression Was found in the liver of treatment group while it Was not found in the liver of DM group.Conclusion Injection of MafA and liposomes at the volume ratio of 1:1 via the portal vein can effectively decrease blood glucose in diabetic rats.
