Chemiluminescence determination of melamine with Luminol-K3Fe（CN）6 system
- VernacularTitle:Chemiluminescence determination of melamine with Luminol-K3Fe（CN）6 system
- Author:
Shuangxiao TANG
;
Xiyan SHI
;
Yuhai TANG
;
Zhongjin YUE
;
Qiqi HE
- Publication Type:Journal Article
- Keywords:
chemiluminescence;
flow injection;
melamine;
urine;
plasma
- From:
Journal of Pharmaceutical Analysis
2011;01(2):104-107
- CountryChina
- Language:Chinese
-
Abstract:
A sensitive chemiluminescence（CL） method was developed for determining melamine in urine and plasma samples based on the fact that melamine can remarkably enhance the chemiluminescence of Luminol-K3 Fe（CN）6 system in alkaline medium. The determination conditions were optimized. Under optimum conditions, the chemiluminescence intensity had a good linear relationship with melamine in the range of 9.0 × 10^-9 - 7.0 × 10^-6 g/mL with a correlation coefficient of 0. 9992. The detection limits （3o） were 3.54 ng/mL for urine sample and 6.58 ng/mL for plasma sample. The average recoveries of melamine were 102.6% for urine sample and 95.1% for plasma sample. Melamine in samples was extracted with liquid-liquid extraction procedures and the assay results coincided very well with that determined with flow injection chemiluminescence method. The method provides a reproducible and stable approach for sensitive detection and quantification of melamine in urine and plasma samples.
