Response surface optimization of microwave-assisted extraction for HPLC-fluorescence determination of puerarin and daidzein in Radix Puerariae thomsonii
- VernacularTitle:Response surface optimization of microwave-assisted extraction for HPLC-fluorescence determination of puerarin and daidzein in Radix Puerariae thomsonii
- Author:
Yingkun LIU
;
E YAN
;
Hanying ZHAN
;
Zhiqi ZHANG
- Publication Type:Journal Article
- Keywords:
microwave-assisted extraction（MAE）;
response surface methodology（RSM）;
puerarin;
daidzein;
HPLC-fluorescence detection;
Radix Puerariae thomsonii
- From:
Journal of Pharmaceutical Analysis
2011;01(1):13-19
- CountryChina
- Language:Chinese
-
Abstract:
Microwave-assisted extraction was optimized with response surface methodology for HPLC-fluorescence determination of puerarin and daidzein in Radix Puerariae thomsonii.The optimized extraction procedure was achieved by soaking the sample with 70% methanol（1∶15,v/v）for 30 min,and then microwave irradiation for 11 min at a power of 600 W.Coupling the extraction process with HPLC-fluorescence presented good recovery,satisfactory precision,and good linear relation.Compared with a method from the Chinese Pharmacopoeia,the proposed method enables higher extraction efficiency and more accurate analytical results.It can be of potential value in quality assessment of Radix Puerariae thomsonii medicinal materials.
