THE CLONING OF HUMAN NEUROTROPHIN-3 GENE
- Author:
Zhihong DONG
;
Huimin REN
;
Haitao HU
;
Yong LIU
;
Quanying WANG
;
Guangxiao YANG
- Publication Type:Journal Article
- Keywords:
human neurotrophin-3(hNT-3);
gene cloning;
polymerase chain reaction (PCR);
DNA sequencing
- From:
Journal of Pharmaceutical Analysis
1999;11(1):8-13
- CountryChina
- Language:Chinese
-
Abstract:
In the present study, we have cloned the gene of human neurotrophin-3 (hNT-3) from the genomic DNA of white blood cells (WBC) by polymerase chain reaction (PCR). The amplification products were cloned into pUC19 and sequenced. Genomic sequence comparison of the cloned fragment and the reported hNT-3 (GenBank M61180) reveals 7 base differences: 1 in the signal peptide, 3 in the prepro peptide, and 3 in the mature hNT-3. Except the 2 varied bases (16th, T to G; 285th, A to C) in the signal peptide and pro-sequence resulted in the change of their encoded amino-acids (Tyr→Asp; Gln→His), the other varied bases have no influence on their respective encoded amino-acids, and all the changes have no influence on the open reading frame (ORF) of the hNT-3.
