A GROWTH INHIBITOR SECRETED FROM CULTURED RABBIT SMOOTHMUSCLE CELLS IS DISTINCT FROM TGF-β
- Author:
Cangbao XU
;
Yaping ZHANG
;
Yawen WANG
;
Yuhai TANG
;
Nianzu CHEN
- Publication Type:Journal Article
- Keywords:
smooth muscle cells;
TGF-β;
growth inhibitor;
conditioned medium
- From:
Journal of Pharmaceutical Analysis
2000;12(1):12-14
- CountryChina
- Language:Chinese
-
Abstract:
Objective To ascertain whether the growth inhibitor in conditioned medium from cultured rabbit arte rial cells is distinct from TGF-β. Methods Rabbit aortic smooth muscle ceils were grown from explained segments of the aorta. Conditioned medium from cultured rabbit aortic smooth muscle ceils and anti-TGF-β were employed in this study. Smooth muscle cell proliferation was measured by XTT detection (Boehringer Mannheim). Results Acidified conditioned medium from smooth muscle ceils had significantly stronger effects of growth inhibition than controls, and anti-TGF-β did not affect the growth inhibitory effect of conditioned medium from cultured rabbit arterial smooth muscle cells. Conclusion The growth inhibiting substance in conditioned medium from cultured rabbit aortic smooth muscle cells is distinct from TGF-β.
