THE IMPROVEMENT OF RT-PCR TECHNIQUE ON DETECTING ROTAVIRUS
- Author:
Yuhai TANG
;
Yun LIU
;
Ying WANG
;
Weili LIANG
;
Junyang WANG
;
Xinglong WANG
- Publication Type:Journal Article
- Keywords:
rotavirus(RV);
RT-PCR;
ELISA
- From:
Journal of Pharmaceutical Analysis
2000;12(1):85-86
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a speed and effective method to detect rotavirus. Methods Using ELISA and one step RT-PCR to detect 196 clinic samples from Xi'an area. Results Compared with ELISA method, one step RT PCR was more sensitive and specific (P ＜0.05). Conclusion One step RT-PCR is a simple, speed, sensitive and spe cific method for clinic and epidemic studies of rotavirus.
