Hypoxia-induced enhancement of cell invasiveness in SMMC7721 hepatocellular carcinoma cells
- Author:
Xue YANG
;
Cheng GUO
;
Lei ZHANG
;
Xin ZHENG
;
Wei YANG
;
Qingguang LIU
- Publication Type:Journal Article
- Keywords:
KAI1/CD82;
hepatocellular carcinoma cell;
hypoxia;
invasion
- From:
Journal of Pharmaceutical Analysis
2009;21(4):252-255
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of hypoxia (1% O2) on the ability of cell invasiveness and expression of KAI1/CD82 in SMMC7721 hepatocellular carcinoma cells. Methods SMMC7721 hepatocellular carcinoma cells were cultured by hypoxia ( 1% O2) in vitro, and the ability of cell invasiveness was analyzed by cell invasion assay.Immunohistochemistry staining technique was used to evaluate the protein expression of KAI1/CD82. Results Cell invasion assay revealed that hypoxia enhanced the ability of invasiveness of hepatocellular carcinoma cells. In addition,KAI1/CD82 protein expression was positive in cultured SMMC7721 hepatocellular carcinoma cells, and it was located diffusedly in the cytoplasm and on the membrane. KAI1/CD82 protein expression was down-regulated when mediated by hypoxia; at the same time, it showed a time-effect relationship. Conclusion Hypoxia can enhance invasiveness of hepatocellular carcinoma cells. The down-regulation of KAI1/CD82 expression may play a certain role in those courses.
