Detection of Campylobacter jejuni by loop-mediated isothermal amplification method
10.7644/j.issn.1674-9960.2017.04.014
- VernacularTitle:通过环介导恒温扩增技术检测空肠弯曲杆菌
- Author:
Shirui QI
;
Huan LI
;
Jun CHEN
;
Weishi LIN
;
Xiangna ZHAO
;
Xiao WEI
;
Erna LI
;
Boxing LI
;
Yanyan MA
;
Weiwei CHENG
;
Jing LU
;
Jing YUAN
;
Gang SUN
- Keywords:
hipO gene;
loop-mediated isothermal amplification;
rapid diagnosis;
Campylobacter jejuni
- From:
Military Medical Sciences
2017;41(4):306-309,317
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a loop-mediated isothermal amplification method for detection of Campylobacter jejuni.Methods Six sets of primers were designed to recognize Campylobacter jejuni specific gene hipO.One was selected as the optimal primer and its specificity and sensitivity to Campylobacter jejuni were evaluated by LAMP reaction in 60 minutes at 62℃.Results The results recorded by the turbidity meter showed that the sensitivity of LAMP with a detection limit of 6.97×102 copies/μl was ten times that of PCR.Conclusion LAMP is a potential and valuable method of detection of Campylobacter jejuni due to its rapidity,simplicity,low cost and accuracy.It is especially suitable for grass-roots medical units.
