Establishment of an animal model by placing one end of PICC in hepatic portal vein of a Beagle dog and leaving the other end out of its body
10.7644/j.issn.1674-9960.2017.04.015
- VernacularTitle:比格犬肝门静脉置管并体外留置模型的建立
- Author:
Baisheng SUN
;
Zheng XUE
;
Yuezhong HE
;
Yunzhi FA
;
Yefeng QIU
;
Zhan YANG
;
Lei ZHANG
;
Yuhao PEI
- Keywords:
Beagles;
inhalation anesthesia;
animal model;
hepatic portal vein
- From:
Military Medical Sciences
2017;41(4):310-312
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an animal model by placing one end of PICC in the hepatic portal vein of a beagle dog and leaving the other end out of its body.Methods Six Beagle dogs were given respiration anesthesia through orotracheal intubation.An incision was made through the right rectus abdominalis to locate the superior mesenteric vein (SMA) and the main hepatic portal vein.The left branch of SMA was separated and cut to put PICC into the main hepatic portal vein before being ligated and fixed.The other end of PICC was elicited through the right abdominal wall and passed beneath the skin to the back neck and fastened in case of movement.Results The anesthetic effect was good and all the operations were successful.The mean operation time was about an hour and the mean blood loss was about 15 ml.The incision healed 5-7 d after operation.Conclusion The establishment of the model can improve the effects of liver-targeting drugs,which can cut down the dosage,lower the cost of treatment and experiment and reduce the adverse effect of medicines.Through PICC,we can directly draw blood from the hepatic portal vein to measure the blood concentration before the first pass elimination.Then according to the concentration,we can calculate the absorption rate in the gastrointestinal tract,which can facilitate related experimental studies.
