Construction and Effect of Artificial MicroRNA Targeted on Hepatitis B Virus
- VernacularTitle:靶向乙型肝炎病毒的外源性microRNA的构建及作用
- Author:
Zuohua CHI
;
Aimei LIU
;
Yan SUN
- Keywords:
nicroRNA;
hepatitis B virus;
replication;
RNA interference
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2017;38(4):489-493
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To investigate the construction and inhibitory effects of artificial microRNA targeted on hepatitis B virus.[Methods] Four artificial microRNA sequences for HBV gene were designed with Invitrogen online design software (BLOCK-iTTM RNAi Designer),amiRNA eukaryotic expression plasmid were constructed and transfected into human hepatocellular carcinoma cell line HepG2.2.15.Hepatitis B virus DNA in supernatant was detected by quantitative fluorescence PCR.HBsAg and HBeAg detected by ELISA.[Results] All of the four artificial microRNA sequences could down regulate the level of HBV-DNA,HBsAg and HBeAg.miR3 had the most significant effect on inhibiting HBV-DNA replication.[Conclusion] Artificial microRNA sequences can effectively inhibit the replication of HBV in vitro.
