MiRNA-374 Inhibits Glioma Cell Growth, Invasion Via Direct Targeting Pituitary Tumor Transforming Gene
- VernacularTitle:miRNA-374靶向PTTG基因抑制胶质瘤细胞的增殖和侵袭能力
- Author:
Yuan ZHAN
;
Yunlong SHEN
;
Yuting CUI
;
Hezhen LI
;
Yong YANG
;
Xuejuan WANG
;
Lihong LIU
- Keywords:
glioma;
miR-374;
PTTG
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2017;38(4):498-504
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To investigate the possible effect of microRNA-374 (miR-374) expression on tumor cells' proliferation and invasion and particular mechanism.[Methods] MiR-374 overexpression lentiviral vector (Lv-miR-374) and a control lentiviral empty vector (LEV) were stably transfected into human glioma U251 and U87 cells,to evaluate the effect of miR-374 on cell proliferation and invasion ability.Target relationship between miR-374 and PTTG were researched by dual luciferase report gene assay.Expression level of correlative signaling pathways of the downstream gene protein was analyzed by Western blot.[Results] We revealed that the overexpression of miR-374 dramatically suppressed glioma cell growth and invasion in vitro.Target relationship between miR-374 and PTTG was confirmed by dual luciferase report gene assay.And decreased protein expression of PTTG,bFGF,AKT,MMP2,and p70S6K was consistent with the effect of miR-374 overexpression.[Conclusion] Decreased miRNA-374 is an unfavorable prognosis marker and promotes glioma cell growth and invasion via direct targeting PTTG.Our findings provide new insights into the role of miR-374 in the development of gliomas,and implicate the potential application of miR-374 in cancer therapy.
