Involvement Endoplasmic Reticulum Stress on Process of Nonalcoholic Fatty Liver Disease Induced by MCD
- VernacularTitle:内质网应激参与蛋氨酸-胆碱缺乏饮食诱导的小鼠非酒精性脂肪肝的形成
- Author:
Wanjun FANG
;
Xu CHEN
;
Wenhua LING
- Keywords:
nonalcoholic fatty liver disease;
nonalcoholic steatohepatitis score;
endoplasmic reticulum stress
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2017;38(4):505-511
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To investigate the effect of the ER stress on the process of nonalcoholic fatty liver disease which is induced by methionine-choline deficient diet.[Methods] C57/BL mice were randomly divided into 8 groups (n =10),group 1-4was given normal diet,5-8 group was given MCD diet.Then took one group from both normal diet and MCD diet in the 2nd,4th,6th,8th week,recorded each group's body weight and liver weight.The liver fibrosis and inflammation in mice were analyzed by histopathological staining and NAS.The expressions of endoplasmic reticulum stress markers such as p-PERK,p-eIF2α,CHOP were detected by Western Blot and real time fluorescent quantitative PCR (q-PCR).[Results] ①The ALT and AST in serum,TG and TC in liver tissue were both higher in MCD group,the same as NAS;②The expressions of PERK,eIF2α,CHOP were significantly higher than the nommal diet group,while the GADD34 expression was lower.What's more,as time went by,the difference was more obvious;3.There was a significant correlation between the ERS index and NAS score in MCD group.[Conclusion]The liver damage and inflammatory response was lifted gradually in the mice fed with MCD diet.The ER stress may take an important role in this process.
