Association of rs10181656 Polymorphism in Signal Transducer and Activator of Transcription 4 Gene with Susceptibility to Unexplained Recurrent Spontaneous Abortion
- VernacularTitle:STAT4基因rs10181656位点的多态性与不明原因复发性自然流产的相关性
- Author:
Yinguang LI
;
Jie LI
;
Jun LIU
;
Jiajie LAI
;
Shanyang HE
;
Zeshan YOU
- Keywords:
recurrent spontaneous abortion;
signal transducer and activator of transcription 4;
polymorphism;
expression
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2017;38(4):551-555
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To investigate associations between the functional polymorphisms of signal transducer and activator of transcription 4 (STAT4) gene and unexplained recurrent spontaneous abortion (URSA) and between STAT4 protein expression and the genotypes of rs 10181656 locus.[Methods] PCR-restriction fragment length polymorphism was used to genotype rs 1 0181656 locus polymorphism in 332 URSA cases and 260 normal controls,in 86 URSA cases and 77 normal controls of which immunohistochemical technique was used to detect STAT4 protein expression.[Results] The frequencies of rs10181656 C/G were 36.45 ％,46.54％ in genotype C/C,46.99％,45.38％ in genotype C/G and 16.57％,8.08％ in genotype G/G between URSA patients and normal controls.They reached statistical difference (P ＜ 0.05).The carriers of rs 10181656 G allele increased the risk of URSA (OR =1.50,P ＜ 0.05).STAT4 protein expression in decidual tissues:①There was statistical difference in the STAT4 protein expression in decidual tissues between cases and controls (P ＜ 0.05).In URSA patients there was statistical difference in the STAT4 protein expression among genotype CC,CG and GG of rs10181656 locus (P ＜ 0.05).So was in normal controls (P ＜ 0.05).In genotype CC there was no difference in the STAT4 protein expression between cases and controls (P ＞ 0.05).Neither was In genotype CG and GG respectively (P all ＞ 0.05).[Conclusion] Functional polymorphisms of the rs10181656 locus could probably associate with the susceptibility of URSA via STAT4 protein expression increased by genotype G/G in maternal decidual tissue.
