Expression and significance of seizure-related DPP-4 and IL-6 in febrile seizures
10.11659/jjssx.03E017086
- VernacularTitle:二肽基肽酶-4和白介素-6在热性惊厥疾病中的表达及意义
- Author:
Jian XU
;
Jian GAO
;
Chengyuan ZHANG
;
Yuanteng FAN
- Keywords:
febrile seizures;
DPP-4;
IL-6;
inflammation
- From:Journal of Regional Anatomy and Operative Surgery
2017;26(8):568-572
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and significances of DPP-4 and IL-6 in febrile seizures(FS).Methods FS were induced in Sprague-Dawley(SD) rats at P14 in a hot water bath by using classical model of hyperthermia-induced seizures.A genome-wide microarray experiment was generated in the rats.The relationship between the differentially expressed genes were analyzed by the method of bioinformatics, and the gene and protein levels of DPP-4 and IL-6 were detected by QPCR,WB and ELISA.Selected 50 children with FS(FS group) and 25 healthy children(control group), and to compare the gene and protein levels of DPP-4 and IL-6 between the two groups.Results Interaction network diagram of differential gene expression showed that there may be interactions between DPP-4 and IL-6.Animal and clinical experiments showed that the DPP-4 and IL-6 gene and protein levels were significantly higher in FS group compared with control group(P<0.05).Conclusion There were high gene and protein expressions of DPP-4 and IL-6 in the FS group compared with the control group.These results indicated that immune and inflammations may play an important role in the FS, and it has provided an attractive pharmacological target for the treatment of FS in clinic.
