A high activity restriction enzyme recognizing GCGC
- Author:
Le Thi Kim Tuyen
- Publication Type:Journal Article
- Keywords:
restriction enzyme
- MeSH:
Enzymes;
Bacteria
- From:Journal of Practical Medicine
2005;512(5):30-32
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
A study was performed to identify a high activity restriction enzyme recognizing 5’-GCGC-3’. Isoschizomer Hhal is a endonucleaza, which has been found in a bacteria isolated from a soil sample picked up in Nghean province. This enzyme has a similar activity with Hha l but it is a other protein of other host bacteria, therefore, it is temporary called a isoschizomer of Hhal. About the enzyme’s specific, Hhal cleaves 5 standard DNA molecules at 384 sites, in which 215 sites at DNA , 103 sites at DNA T7, 18 sites at x174, 31 sites at pBR 322 and 17 sites at pUC 19. Isoschizomer Hhal with high activity, stable met the need of users and laboratory of Vietnam.
