Cell suspension and level of IL-17 in bronchoalveolar lavage fluid (BALF) of children with labor pneumonia combined with mycoplasma pneumonia and its clinical significance
10.3969/j.issn.1007-1989.2016.02.007
- VernacularTitle:合并支原体感染的大叶性肺炎患儿肺泡灌洗液中常规细胞学检测及意义
- Author:
Yunxia WANG
;
Wenguang LIU
;
Qiang GU
- Keywords:
labor pneumonia;
mycoplasma pneumonia infection;
BALF;
conventional cells staining;
interleukin-17;
children
- From:
China Journal of Endoscopy
2016;22(2):30-33
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore cell suspension and level of IL-17 in bronchoalveolar lavage fluid (BALF) of children with labor pneumonia combined with mycoplasma pneumonia and its clinical significance. Methods 87 children with labor pneumonia who had been done BAL were divided into CPIS ≥ 6 group and CPIS < 6 group ac-cording to the clinical pneumonia infection score (CPIS). Meanwhile, according to the results of MP-DNA, we grouped the children above into combined with mycoplasma pneumonia infection group and no mycoplasma pneumo-nia infection group, then combined with mycoplasma pneumonia infection group into group of MP-DNA:103~106 and group of MP-DNA: 106~. ELISA method was used to detect IL-17 level, HE staining and microscopy to sort cells, PCR detect MP-DNA. Results The percentage of neutrophils and lymphocytes in BALF are not related to that in blood ( > 0.05). The percentage of columnar epithelial cells in CPIS < 6 group were higher than CPIS ≥ 6, how-ever, macrophagocyte was lower ( < 0.05). The percentage of neutrophils are related with both MP-DNA ( = 0.48,= 0.000) and IL-17 ( =0.49, =0.000). Conclusions The composition of BALF is more appropriate to react the circumstance of lobar pneumonia. When lobar pneumonia occurs, columnar epithelial cells are damaged, macrophagocyte gathered to participate in the inflammatory response. In Mycoplasma pneumonia infection group, IL-17 raises neutrophils to resist infection, NE%in BALF can indirectly reflect the mycoplasma infection degree.
