Biological Titer Determination of Traditional She Medicine-Gardeniae Radix
- VernacularTitle:畲药山里黄根品质生物效价检测
- Author:
Weidong CHEN
;
Lixian LIU
;
Lijun XIA
;
Shuaiying LIU
- Keywords:
Gardeniae Radix;
Antimicrobial value;
Biological titer;
Turbidity method;
Quality evaluation;
Chikusetsu saponin Ⅳa
- From:
China Pharmacist
2017;20(8):1463-1465
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the antimicrobial effectiveness against traditional She medicine-Gardeniae Radix and determine the titer of Gardeniae Radix.Methods: The antibacterial activity of five standard strains(Escherichia coli , Staphylococcus aureus , Pseudomonas aeruginosa , Candida albicans and Aspergillus niger)was investigated.A method for the biological value evaluation of Gardeniae Radix was established by using a turbidity method, and compared with the content of chikusetsu saponin Ⅳa determined by HPLC.Results: The sensitivity of Gardeniae Radix to Staphylococcus aureus and Escherichia coli was high.The recovery of the turbidity method was within the range of 90%-110%, and the reliability limit rate was less than 5%.The content of chikusetsu saponin Ⅳa in Gardeniae Radix was not completely consistent with the result of the bioassay method.Conclusion: The bioassay method established in the experiment can be one of the methods for the quality evaluation of Gardeniae Radix.
