Simultaneous Determination of Schisantherin, Deoxyschizandrin and Anwuligan in Wuzhi Pellets by HPLC
- VernacularTitle:HPLC法同时测定五酯微丸中五味子酯甲、五味子甲素和安五酯素
- Author:
Ping HUANG
;
Yan WANG
- Keywords:
Wuzhi pellets;
HPLC;
Schisantherin;
Deoxyschizandrin;
Anwuligan
- From:
China Pharmacist
2017;20(8):1499-1501
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method for HPLC determination of schisanthein, deoxyschizandrin and anwuligan in Wuzhi pellets simultaneously and control the quality of Wuzhi pellets.Methods: Schisantherin, deoxyschizandrin and anwuligan were determined by HPLC with a chromatographic column of Welch Ultimate XB-C 18 (150 mm× 4.6 mm , 3 μm), the mobile phase was tetramathylene oxide-water with gradient elution.The detection wavelength was 228 nm.The column temperature was 35℃.Results: The linear range of schisantherin was 0.040-0.805 μg (r=0.999 9),and the average recovery was 97.0% with RSD of 1.67%.The linear range of deoxychizandrin was 0.092-1.846μg (r=0.999 9),and the average recovery was 102.5% with RSD of 1.49%.The linear range of anwuligan was 0.020-0.998 μg (r=0.999 9),and the average recovery was 95.0% with RSD of 1.78% (n =6).Conclusion: The method is simple, accurate and reproducible.It can improve the quality standard of Wuzhi pellets.
