Change of Plasma Xanthine Oxidase Activity by Intermittent Hepatic Ischemia-Reperfusion.
10.4097/kjae.2004.47.5.692
- Author:
Seong Jo HA
1
;
Jae Hoon KIM
;
Sung Sik PARK
;
InKyeom KIM
;
Woonyi BAEK
;
Dong Gun LIM
Author Information
1. Departments of Anesthesiology and Pain Medicine, School of Medicine, Kyungpook National University, Daegu, Korea. dglim@knu.ac.kr
- Publication Type:Original Article
- Keywords:
hepatectomy;
ischemia-reperfusion;
pringle maneuver;
reactive oxygen species;
superoxide;
xanthine oxidase
- MeSH:
Blood Transfusion;
Cytochromes c;
Hemorrhage;
Hepatectomy;
Humans;
Ischemia;
Plasma*;
Reactive Oxygen Species;
Reperfusion;
Reperfusion Injury;
Superoxides;
Xanthine Dehydrogenase;
Xanthine Oxidase*;
Xanthine*
- From:Korean Journal of Anesthesiology
2004;47(5):692-697
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The pringle maneuver (PM), hepatic inflow occlusion, during hepatic surgery reduces intraoperative bleeding and blood transfusion requirement, but hepatic ischemia/reperfusion injury is inevitable. During ischemia, xanthine oxidoreductase is converted to xanthine oxidase (XO), which can serve as a critical source of reactive oxygen species (reduces O2 to O2 .-) that contribute to inflammatory signaling, ischemia-reperfusion injury, and an impaired vascular function. The purpose of the present study was to follow changes of XO activity and O2 .- production during hepatic surgery under PM. METHODS: Eleven patients that underwent hepatectomy under intermittent PM were studied. Blood was withdrawn before PM, and 10 and 20 minutes after final reperfusion. Plasma XO activity was measured using a spectrophotometer after incubating plasma with/without xanthine for one-hour. Superoxide (O2 -) production was followed by measuring by cytochrome c reduction by plasma XO. RESULTS: After final reperfusion, plasma XO activity had increased four-fold (0.36 +/- 0.06 to 1.25 +/- 0.25 mU/ml) with a concomitant increase in O2 .- production (0.66 +/- 0.29 to 1.66 +/- 0.40microM/min). CONCLUSIONS: Significantly more XO is released into the systemic circulation after intermittent PM, with subsequently increased O2 .- production. The significant contribution of XO to hepatic surgery under PM might be beneficially managed using an anesthetic with a known antioxidative effect.
