Effect of MicroRNA-9 on cell proliferation and collagen synthesis in hepatic stellate cells by down-regulation of TGFBR2
10.3760/cma.j.issn.1008-1372.2017.06.010
- VernacularTitle:MicroRNA-9通过下调TGFBR2表达抑制肝星状细胞增殖和胶原合成
- Author:
Rongquan FU
;
Zhiquan MA
;
Yibing HU
;
Danping HU
- Keywords:
MicroRNAs/PD/ME;
Hepatocytes/CY/DE;
Astrocytes/DE;
Cell proliferation/DE;
Collagen/ME
- From:
Journal of Chinese Physician
2017;19(6):833-835
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of MicroRNA-9 (miR-9) on cell proliferation and collagen synthesis in hepatic stellate cells (HSCs),and to explore the potential mechanism.Methods HSC-T6 cells were cultured and transfected with miR-9 mimics with lipofectamine 2000.After incubation 48 h,the cells were collected and total proteins and RNAs were extracted.The expression of miR-9 was detected by quantitative real time polymerase chain reaction (qRT-PCR).The protein expression of type Ⅰ collagen and type Ⅲ collagen were measured by Western blot.The methyl thiazolyl tetrazolium (MTT) method was used to asses the proliferation of HSC-T6 cells.The expression of transforming growth factor-β1 receptor 2 (TGFBR2) was detected by qRT-PCR and Western blot.Results Compared to the control group,miR-9 expression in HSCs was increased in the miR-9 mimics group (P ＜ 0.05),type Ⅰ and type Ⅲ collagen protein expression was reduced by (44 ± 2) ％ and (50 ± 3) ％ (P ＜ 0.01),respectively.The proliferation activity of HSCs was decreased by (48 ± 4)％ (P ＜ 0.05).The expression of TGFBR2 was inhibited in the miR-9 mimics group.Conclusions Upregulation of miR-9 plays a role on suppressing cell proliferation and collagen synthesis in HSCs.This process might be mediated by downregulation of TGFBR2.
