Effect of sevoflurane preconditioning on expression of heat shock protein 70 during renal ischemia-reperfusion in rats
10.3760/cma.j.issn.0254-1416.2017.05.031
- VernacularTitle:七氟醚预处理对大鼠肾缺血再灌注时HSP70表达的影响
- Author:
Jinjun SHU
;
Yumin TANG
;
Yiding CHEN
;
Xiaoni ZHANG
;
Kexian ZHANG
- Keywords:
Anesthetics,inhalation;
Ischemic preconditioning;
Reperfusion injury;
Kidney;
HSP70 heat-shock proteins
- From:
Chinese Journal of Anesthesiology
2017;37(5):629-631
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of sevoflurane preconditioning on the expression of heat shock protein 70 (HSP70) during renal ischemia-reperfusion in rats.Methods Twenty-four pathogen-free healthy adult male Sprague-Dawley rats,weighing 250-300 g,aged 2-3 months,were divided into 3 groups (n=8 each) using a random number table:sham operation group (group S),renal I/R group (group I/R) and sevoflurane preconditioning group (group SP).The animals were anesthetized with 2％ pentobarbital sodium 50 mg/kg.After the right kidney was removed,the left renal artery was clamped for 45 min followed by 2 h reperfusion to establish the model of renal I/R injury.In group SP,2.2％ sevoflurane was inhaled for 1 h followed by 10-min washout before the model was established.The animals were sacrificed at the end of rcpcrfusion,and kidneys were then removed for determination of the expression of HSP70 (by immunohistochemistry) and malondialdehyde (MDA) content in renal tissues (by thiobarbituric acid colorimetric method).Results Compared with group S,the expression of HSP70 in renal tissues was significantly up-regulated,and the MDA content in renal tissues was increased in t/R and SP groups (P＜0.05).Compared with group I/R,the expression of HSP70 in renal tissues was significantly up-regulated,and the MDA content in renal tissues was decreased in group SP (P＜0.05).Conclusion The mechanism by which sevoflurane preconditioning reduces renal I/R injury is related to up-regulation of HSP70 expression in rats.
