Effect of CaMK Ⅱ expression on apoptosis of rat hepatocytes BRL-3A
10.3760/cma.j.issn.0254-1785.2017.04.009
- VernacularTitle:钙调素依赖蛋白激酶家族Ⅱ表达调控对大鼠肝细胞BRL-3A凋亡的影响
- Author:
Jianghua RAN
;
Kepu ZHENG
;
Wang LI
;
Xibing ZHANG
;
Boqiang LIU
- Keywords:
CaMK Ⅱ;
Rat hepatocytes;
BRL-3A;
Lentivirus;
Cell apoptosis
- From:
Chinese Journal of Organ Transplantation
2017;38(4):239-243
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of CaMK Ⅱ expression on apoptosis of rat hepatocytes BRL-3A.Methods Rat BRL-3A cells were stable passage were cultured.The CaMK Ⅱ γ protein (LV-CaMK Ⅱ γ group) and CaMK Ⅱ γshRNA (shRNA group) lentiviral expression systems were constructed.The corresponding blank vectors (LV-NC group and shRNA-NC group) and normal saline (CON group) were perfused into the control groups.The expression levels of CaMK Ⅱ,Cyt C and MF proteins were detected by Western blotting,and the apoptosis rate of BRL-3A cells was measured by Tunel method.Results The protein expression of CaMK Ⅱ,Cyt C and AIF in LV-CaMK Ⅱ γ group was significantly higher than that in CON group (P＜0.05).The protein expression of CaMK Ⅱ,Cyt C and AIF in shRNA group was significantly lower than that in CON group (P＜ 0.05).There was no significant difference among CON group,LV-NC group and shRNA-NC group (P＞0.05).At the same time point,the apoptosis rate of hepatocytes in LV-CaMK Ⅱ γ group was significantly higher than that in CON group (P＜0.05).At the same time point,the apoptosis rate of hepatocytes in shRNA group was significantly higher than in CON group (P＜0.05).There was no significant difference in the apoptosis of hepatocytes among CON group,LV-NC group and shRNA-NC group (P＞0.05).Conclusion The specific CaMK Ⅱ signaling pathway can inhibit the apoptosis of BRL-3A cells,while the enhanced CaMK Ⅱ signaling pathway promotes the apoptosis of BRL-3A cells.
