Study on the Effect and Its Mechanism of Fuzheng Jiedu Quyu Formula Combined with Oxaliplatin on Proliferation and Apoptosis of Human Colon Cancer HT-29 Cell
10.6039/j.issn.1001-0408.2017.19.06
- VernacularTitle:扶正解毒祛瘀方联合奥沙利铂对人结肠癌HT-29细胞增殖与凋亡的影响及机制研究
- Author:
Xiumei ZHAO
;
Bing ZHOU
;
Guixian ZHANG
;
Zhongqiu CHAI
;
Xiaoyun LIU
;
Hongbin LIU
- Keywords:
Fuzheng Jiedu Quyu formula;
Oxaliplatin;
Human colon cancer HT-29 cell;
Proliferation;
Apoptosis;
in vitro
- From:
China Pharmacy
2017;28(19):2613-2616
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effect and its mechanism of Fuzheng Jiedu Quyu formula(short forFuzheng formula) combined with oxaliplatin (L-OHP) on human colon cancer HT-29 cell proliferation and apoptosis. METHODS:HT-29 cells were divided into blank control group(without drugs),Fuzheng formula group(1000 mg/L),L-OHP group(31.25 mg/L)and combi-nation group (1000 mg/L Fuzheng formula+31.25 mg/L L-OHP). After cultured with corresponding drug for 48 h,MTT method was used to detect the cell proliferation;the changes of cellular morphology were observed by invert microscope;flow cytometry was used to detect the cell cycle and apoptosis rate. Proapoptotic gene Bax,apoptotic gene Bcl-2 mRNA expressions were deter-mined by real-time fluorescence quantitative polymerase chain reaction method;Bax,Bcl-2 protein expressions were assayed by Western blot. RESULTS:Compared with blank control group,cell proliferation was inhibited in L-OHP group and combination group;cell proportion was increased in S stage,G2/M stage and decreased in G0/G1 stage(P<0.05). Cell apoptosis rate in L-OHP group,Fuzheng formula group and combination group was increased;Bax mRNA and protein expression were up-regulated,Bcl-2 mRNA expression was downregulated(P<0.05);and combination group changed more obviously than the single drug groups(P<0.05). CONCLUSIONS:Fuzheng formula combined with L-OHP can inhibit HT-29 cell proliferation and promote its apoptosis, showing better effects than either of the two drugs alone. The mechanism may be associated with up-regulation of Bax gene and pro-tein expressions and down-regulation of Bcl-2 gene expressions in cells.
