Improvement Effect of Eucalyptol Enteric Soft Capsule on Lipopolysaccharide-induced Acute Lung Injury in Mice
10.6039/j.issn.1001-0408.2017.19.18
- VernacularTitle:桉柠蒎肠溶软胶囊对脂多糖诱导小鼠急性肺损伤的改善作用
- Author:
Xuan LI
;
Yumei YANG
- Keywords:
Eucalyptol enteric soft capsule;
Acute lung injury;
Lipopolysaccharide;
Mice;
Improvement effect
- From:
China Pharmacy
2017;28(19):2655-2658
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the improvement effect of Eucalyptol enteric soft capsule on mice with lipopolysaccha-ride(LPS)-induced acute lung injury(ALI). METHODS:60 mice were randomly divided into blank control group,model control group,Eucalyptol enteric soft capsule low-dose,medium-dose,high-dose groups(100,300,900 mg/kg),12 in each group. Mice in administration groups were intragastrically given relevant medicines,mice in blank control group and model control group were intragastrically given equal volume of normal saline(0.1 mL/10 g). After 2 h of administration,except for the blank control group, ALI was induced in other groups by atomized LPS. After 6 h of modeling,the mice were sacrificed,alveolar lavage fluid(BALF) and lung tissue were taken. Morphological changes of lung tissue were observed under microscope;number of total cells,neutro-phils in BALF were calculated by blood cell count plate and staining by wright-giemsa respectively. Total protein concentration in BALF supernatant was detected by BCA method;TNF-α,IL-6 contents in BALF supernatant were determined by enzyme-linked immunosorbent assay. RESULTS:Compared with blank control group,lung tissue of mice in model control group showed obvious pathological damage and severe pulmonary edema;number of total cells,neutrophils in BALF,total protein concentration and TNF-α,IL-6 contents in BALF supernatant were significantly increased(P<0.01). Compared with model control group,pathologi-cal damage in lung tissue of mice was obviously improved in Eucalyptol enteric soft capsule high-dose group,number of total cells,neutrophils in BALF,total protein concentration and TNF-α,IL-6 contents in BALF supernatant were significantly decreased (P<0.05);and there were no significant differences in other groups (P>0.05). CONCLUSIONS:High-dose Eucalyptol enteric soft capsule can obviously improve LPS-induced ALI of mice.
