Optimization of Methanol Extraction Technology for Tripterine in the Fruit of Celastrus monospermus by Orthogonal Design
10.6039/j.issn.1001-0408.2017.19.24
- VernacularTitle:正交试验优化独子藤果实中雷公藤红素的醇提工艺
- Author:
Qirong XU
;
Qiong ZHOU
;
Zhiping ZHANG
;
Jiu CHEN
- Keywords:
Celastrus monospermus;
Tripterine;
Single factor;
Orthogonal test;
HPLC
- From:
China Pharmacy
2017;28(19):2677-2679
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To optimize the methanol extraction technology of tripterine in the fruit of Celastrus monospermus. METHODS:HPLC was adopted to determine the content of tripterine. Using extraction time,extraction times,solid-liquid ratio as investigation factors,extraction rate of tripterine as investigation index,orthogonal test was designed,and verification test was con-ducted. RESULTS:The optimal methanol extraction technology was as follow as 45% methanol extracting 2 h each time with sol-id-liquid ratio of 1:8 for 3 times. In the verification test,the average extraction rate of tripterine was 0.917 mg/g(RSD=2.85%, n=3). CONCLUSIONS:The optimized methanol extraction technology is stable and feasible with high extraction rate,and is suit-able for the extraction of tripterine in the fruit of C. monospermus.
