The Role of CBS in Injury Time Estimation after Brain Contusion
10.3969/j.issn.1004-5619.2017.03.001
- VernacularTitle:CBS在脑挫伤后损伤时间推断中的作用
- Author:
Yang CHU
;
Guoxian HAN
;
Yaoqi WANG
;
Haiyan SHAN
;
Xiping CHEN
;
Luyang TAO
;
Mingyang ZHANG
- Keywords:
forensic pathology;
brain injuries;
cystathionine beta-synthase;
injury tim e;
m ice
- From:
Journal of Forensic Medicine
2017;33(3):221-224,231
- CountryChina
- Language:Chinese
-
Abstract:
Objective T o observe the changes of cystathionine β-synthase (C B S ) expression in the cere-bral cortex after brain contusion at different tim es. Methods A n experim ental m odel of traum atic brain injury (T B I) in m ice w as established by an im proved w eight-drop device. T hen W estern blotting and im m unohistochem ical exam ination w ere used to detect the C B S expression in cerebral cortex around in-jury at different tim e points (1 h, 6 h, 12 h, 1 d, 2 d, 3 d, 7 d). Results T he results of W estern blotting revealed that the expression level of C B S w as dow n-regulated and reached its low est level at the 3rd days after injury, and then restored to norm al level after 7 days. T he results of im m unohistochem istry show ed that C B S w as present in the norm al brain cortex. C B S expression gradually decreased at the 3rd days after injury, and then restored to norm al level after 7 days. Conclusion C B S has the potential to be a reference index for tim e estim ation after brain contusion in forensic practice.
