Sixty-six Biallelic Genetic Markers on Y chromosome by MALDI-TOF-MS
10.3969/j.issn.1004-5619.2017.03.005
- VernacularTitle:MALDI-TOF-MS对Y染色体上66个二等位基因的检测
- Author:
Yutong SONG
;
Li LI
;
Linan ZHANG
;
Ruxin ZHU
;
Yan LIU
;
Yuan LIN
- Keywords:
forensic genetics;
Y chrom osom e;
polym orphism,genetic;
m atrix-assisted laser desorption-ionization;
single nucleotide polym orphism;
insertion/deletion
- From:
Journal of Forensic Medicine
2017;33(3):239-243
- CountryChina
- Language:Chinese
-
Abstract:
Objective T o analyse the genetic polym orphism s of 66 biallelic genetic m arkers on Y chro-m osom e in E astern C hinese H an population, and evaluate their values in forensic application. Methods G enotyping of 66 biallelic genetic m arkers on Y chrom osom e w as studied in 205 unrelated m ales of E astern C hinese H an population by m ultiplex PC R com bined m atrix-assisted laser desorption/ionization tim e-of-flight m ass spectrom etry (M A L D I-T O F-M S ). T he allele frequencies on the loci to be tested w ere calculated by direct counting m ethod, and the gene diversity (G D ) and haplotype diversity (H D ) w ere calculated by corresponding form ulas. T he haplotypes of this system w ere tested by softw are A rlequin v3.5.2.2 and the com parison of population genetics w ere analyzed. Results A total of 60 biallelic genetic m arkers on Y chrom osom e w ere polym orphic in m ales of E astern C hinese H an population, and the ranges of G D w ere from 0.0385 to 0.5019. E ighty-five different haplotypes w ere observed and the H D w as 0.9703. T he differences of partial SN P loci betw een the H an population of E astern C hina and that of X injiang and G uangdong w ere statistically significance. Conclusion Sixty biallelic genetic m arkers and the detection system can com plem entally provide genetic inform ation in kinship testing and individual identification. T he M A L D I-T O F-M S technology is able to type biallelic genetic m arkers.
