Genotyping of ABO Blood Group in Partial Population of Yunnan Province by SNaPshot Technology
10.3969/j.issn.1004-5619.2017.03.013
- VernacularTitle:SNaPshot技术应用于云南省部分人群ABO血型基因分型
- Author:
Shuxin YU
;
Faming ZENG
;
Yanzhang JIN
;
Hongjing WAN
;
Dian ZHAI
;
Yuming XING
;
Baowen CHENG
- Keywords:
forensic genetics;
A B O blood group system;
genetic testing;
SN aPshot technology
- From:
Journal of Forensic Medicine
2017;33(3):277-280
- CountryChina
- Language:Chinese
-
Abstract:
Objective T o detect the genotype of A B O blood group by SN aPshot technology. Methods D N A w ere extracted from the peripheral blood sam ples w ith know n blood groups (obtained by serology) of 107 unrelated individuals in Y unnan. Six SN P loci of the 261th, 297th, 681th, 703th, 802th, and 803th nucleotide positions w ere detected by SN aPshot M ultiplex kit, and relevant genetics param eters w ere cal-culated. Results In 107 blood sam ples, the allele frequencies of types A , B , O A, and O G w ere 0.3551, 0.1682, 0.2300 and 0.2476, respectively, w hile that of types A G and cis A B w ere not detected. T he geno-typing results of A B O blood group w ere consistent w ith that of serologic testing. Conclusion SN aPshot technology can be adapted for genotyping of A B O blood group.