Genotyping of ABO Blood Group in Partial Population of Yunnan Province by SNaPshot Technology

VernacularTitle:SNaPshot技术应用于云南省部分人群ABO血型基因分型
Author: Shuxin YU ; Faming ZENG ; Yanzhang JIN ; Hongjing WAN ; Dian ZHAI ; Yuming XING ; Baowen CHENG
Keywords: forensic genetics; A B O blood group system; genetic testing; SN aPshot technology
From: Journal of Forensic Medicine 2017;33(3):277-280
CountryChina
Language:Chinese
Abstract: Objective T o detect the genotype of A B O blood group by SN aPshot technology. Methods D N A w ere extracted from the peripheral blood sam ples w ith know n blood groups (obtained by serology) of 107 unrelated individuals in Y unnan. Six SN P loci of the 261th, 297th, 681th, 703th, 802th, and 803th nucleotide positions w ere detected by SN aPshot M ultiplex kit, and relevant genetics param eters w ere cal-culated. Results In 107 blood sam ples, the allele frequencies of types A , B , O A, and O G w ere 0.3551, 0.1682, 0.2300 and 0.2476, respectively, w hile that of types A G and cis A B w ere not detected. T he geno-typing results of A B O blood group w ere consistent w ith that of serologic testing. Conclusion SN aPshot technology can be adapted for genotyping of A B O blood group.