Individual Identification of Cartilage by Direct Amplification in Mass Disasters
10.3969/j.issn.1004-5619.2017.03.014
- VernacularTitle:直接扩增法用于重大灾难事故中软骨的个体识别
- Author:
Chuanhai WANG
;
Cheng XU
;
Xiangqin LI
;
Yong WU
;
Zhou DU
- Keywords:
forensic genetics;
cartilage;
genotyping techniques;
direct am plification;
individual identification
- From:
Journal of Forensic Medicine
2017;33(3):281-283
- CountryChina
- Language:Chinese
-
Abstract:
Objective T o explore the effectiveness of direct am plification for the ST R analysis of carti-lage, and to accelerate the effectiveness of disaster victim identification. Methods E ighty-eight cartilage sam ples w ere directly am plified by Pow erPlex誖21 kit, and the results of genotyping w ere com pared w ith that obtained by the m agnetic beads m ethod. Results In 88 cartilage sam ples, the ST R genotypes w ere successfully detected from 84 sam ples by direct am plification and m agnetic beads m ethod, and both the results of genotyping by tw o m ethod w ere consistent. Conclusion D irect am plification w ith Pow er-Plex誖21 kit can be used for ST R genotyping of cartilages. T his m ethod is operated easily and prom ptly, w hich has a potential application in the individual identification of m ass disasters.
