The value of multiple immunological indexes in the identification of anaphylactic death by penicillin
10.13618/j.issn.1001-5728.2017.03.008
- VernacularTitle:多项免疫学指标在青霉素过敏死亡鉴定中的价值
- Author:
Xun XIE
;
Hai XIE
;
Yanxu LU
;
Feihu WANG
- Keywords:
forensic pathology;
anaphylactic shock by penicillin;
immunological methods;
mast cell proteases;
interleukin
- From:
Chinese Journal of Forensic Medicine
2017;32(3):261-265
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression level of tryptase, chymase, IL-4 and IL-10 in guinea pigs died from anaphylactic death caused by penicillin allergy within 48 hours. Methods Guinea pigs were sensitized and elicited by penicilloyl-protein, the blood and tissues were extracted within 48 hours after the death. The expression of tryptase and chymase in tracheas and lungs were detected by the ways of immunohistochemistry, IL-4 and IL-10 levels in serum, tracheas and lungs were detected by ELISA. Results Compared with the control group, the expression of tryptase and chymase has enhanced in lungs and tracheas, the level of IL-4 and IL-10 increased in serum, lungs and tracheas in experimental groups(P<0.05). Conclusion Tryptase, chymase, IL-4 and IL-10 have significant value in the identification of the deaths caused by penicillin allergy.
