Application of mtDNA COI genes in common sarcosaphagous flies species identification
10.13618/j.issn.1001-5728.2017.03.005
- VernacularTitle:应用mtDNA COI基因序列鉴别常见嗜尸性蝇类种属
- Author:
Xuebo LI
;
Chunli DING
;
Feng ZHAO
;
Liangliang LI
;
Qingshan WANG
;
Qiang GONG
;
Xiaojun YU
- Keywords:
forensic entomology;
Sarcosaphagous flies;
mtDNA;
COI;
species identification
- From:
Chinese Journal of Forensic Medicine
2017;32(3):249-252
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the application of mtDNA COI genes in common sarcosaphagous flies species identification. Methods 30 sarcosaphagous fly samples were indentified by morphological method which collected in different regions belonging to 2 families, 4 genera and 6 species. MtDNA was extracted for the PCR amplification reaction in COI gene. The PCR products were purified through agar gel electrophoresis and sequenced. Sequences of 498 bp in COI gene were disposed by multiple-alignment software of DNAMAN. Sequences divergence of 498 bp between and within species of COI gene were processed by software of MEGA. Results It was showed that there is a certain sequence differences between the 30 samples from 6 species. The intraspecific and interspecific divergence of sequence variation ranged from 0.1% to 1.6% and 2.2% to 11.2% respectively. All the species can be identified successfully by this method. Conclusion Species identification of sarcosaphagous flies can be conducted by sequence analysis and phylogenetic tree of COI gene. This method can be effectively used in fast and accurate identification in forensic entomology.
