Methodology establishment of detection of Vibrio vulnificus by flow cytometry and aptamer
10.3969/j.issn.1005-1678.2017.07.001
- VernacularTitle:核酸适配体联合流式细胞术定量检测创伤弧菌方法的建立
- Author:
Dejing LIU
;
Bo HU
;
Dingfa PENG
;
Shengqun OUYANG
;
Lianghua WANG
;
Binghua JIAO
- Keywords:
aptamer;
Vibrio vulnificus;
flow cytometery;
pathogen detection
- From:
Chinese Journal of Biochemical Pharmaceutics
2017;37(7):1-5
- CountryChina
- Language:Chinese
-
Abstract:
Objective Establish detection method to measure Vibrio vulnificus rapidly and accurately.MethodsUsing flow cytometry(FCM)and a 5'-FITC fluorescent labeled aptamer with high binding affinity to detect Vibrio vulnificus rapidly.Measure a series of concentrations of Vibrio vulnificus to identify the Limit of Blank, Lower Limit of Detection, Linearity Range, etc.ResultsCombined application of FCM and the aptamer can detect Vibrio vulnificus rapidly with the duration less than 1 hour and lower limit of detection as low as 29 CFU/mL.Conclusion The aptamer targeting Vibrio vulnificus is an excellent detective element, while FCM can realize accurate quantitative detection.The detection method has great application potential.
