Cytoprotective Effect of Polyphenolic Compounds against Oxidative Stress in Cultured Retinal Pigment Epithelial Cells.
10.3341/jkos.2016.57.1.106
- Author:
Kyung Hoon SEO
1
;
Seung Young YU
;
Hyung Woo KWAK
Author Information
1. Department of Ophthalmology, Kyung Hee University Medical Center, Kyung Hee University School of Medicine, Seoul, Korea. hwkwak@khu.ac.kr
- Publication Type:Original Article
- Keywords:
Grape seed extract;
Oxidative stress;
Polyphenols;
Proanthocyanidins;
Retinal pigment epithelium
- MeSH:
Adult;
Cell Death;
Cell Survival;
Epithelial Cells*;
Fluorescence;
Free Radicals;
Grape Seed Extract;
Humans;
Hydrogen Peroxide;
Mortality;
Oxidative Stress*;
Polyphenols;
Proanthocyanidins;
Reactive Oxygen Species;
Retinal Diseases;
Retinal Pigment Epithelium;
Retinaldehyde*;
Vitis
- From:Journal of the Korean Ophthalmological Society
2016;57(1):106-112
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Grape seed-derived polyphenols (GSPs) provide a concentrated source of polyphenols having antioxidant capacity. In this study we investigated the cytoprotective effect of GSP against oxidative stress-induced cell damage in cultured human retinal pigment epithelial (RPE) cells. METHODS: Cultured adult retinal pigment epithelium (ARPE)-19 cells were incubated with GSP from Vitis vinifera (0.1, 0.5, 1, 5 or 10 microg/mL) for 24 hours and treated with hydrogen peroxide (H2O2, 0.4 mM) for 24 hours to induce oxidative stress. Cell viability was measured using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Intracellular reactive oxygen species (ROS) was quantified using 2',7'-dichlorofluorescein diacetate (DCF-DA) fluorescence. RESULTS: The percentage of viable RPE cells was significantly lower in cultures treated with H2O2 0.4 mM than in control cultures. GSP significantly reduced H2O2-induced cell death in a dose dependent manner. GSP at 0.1, 0.5, 1, 5 and 10 microg/mL significantly reduced cell mortality due to the treatment with H2O2. Intracellular ROS production increased significantly in cultures treated with H2O2 0.4 mM compared with control. There was a significant dose-dependent decrease in intracellular ROS levels after treatment of RPE with GSP. CONCLUSIONS: GSP, a natural polyphenolic compound, can protect RPE cells from H2O2-induced oxidative stress and reduce intracellular ROS production by scavenging free radicals. This suggests potential effects of polyphenolic compounds against retinal diseases associated with oxidative stress.
