Neuroprotective Effects of Betaxolol Mediated by Heme Oxygenase-1 Induction in RGC-5.
10.3341/jkos.2016.57.1.113
- Author:
Jae Bong CHA
1
;
Min Young KWON
;
Su Wol CHUNG
;
Je Moon WOO
Author Information
1. Department of Ophthalmology, Ulsan University Hospital, University of Ulsan College of Medicine, Ulsan, Korea. limbus68@naver.com
- Publication Type:Original Article
- Keywords:
Betaxolol;
Heme oxygenage-1;
Hypoxia;
Retinal ganglion cells
- MeSH:
Anoxia;
Betaxolol*;
Blotting, Western;
Cell Survival;
Heme Oxygenase-1*;
Heme*;
Incubators;
Neuroprotective Agents*;
Retinal Ganglion Cells;
Zinc;
Brimonidine Tartrate;
Travoprost
- From:Journal of the Korean Ophthalmological Society
2016;57(1):113-119
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To evaluate the neuroprotective effects of betaxolol (betaxolol hydrochloride) under hypoxic conditions using retinal ganglion cells (RGC-5) and determine whether heme oxygenase-1 (HO-1) expression exerts cytoprotective effects. METHODS: In this study, cultured RGC-5 cells were incubated with different concentrations of betaxolol hydrochloride (0.1 microM, 1 microM or 5 microM) and with 10 microM zinc protoporphyrin (ZnPP), in a hypoxia incubator (1% O2, 5% CO2, 94% N2) for 48 hours and the cell viability of each group was determined. Additionally, cell viability was measured after RGC-5 cells were incubated with 5 microM of brinzolamide (Azopt(R)), brimonidine tartrate (Alphagan(R)) or travoprost (Travatan(R)). RGC-5 cells were divided into three groups and incubated under three different conditions, normoxia group (20% O2, 5% CO2), hypoxia group (1% O2, 5% CO2) and the group with 5 microM of Betoptic S(R) treated under hypoxic conditions (hypoxia, Betoptic S(R)). After incubation for 4, 8, 12 and 24 hours, HO-1 expression was analyzed using Western blotting. RESULTS: Cell viability significantly increased in RGC-5 cells treated with Betoptic S(R) compared with other antiglaucoma agents. Increased levels of HO-1 expression indicate its relevance in cell viability. Furthermore, increased RGC-5 cell viability by Betoptic S(R) was significantly reduced in the HO-1 inhibitor ZnPP-treated group. CONCLUSIONS: We reaffirmed the known cytoprotective effects of Betoptic S(R) and the results suggests that HO-1 expression exerts cytoprotective effects against hypoxia.
