Effects of ARHI gene transfection on chemokines and receptors related gene expression profile of PANC1 cells
10.3760/cma.j.issn.1674-1935.2017.03.007
- VernacularTitle:ARHI基因转染胰腺癌PANC1细胞后对趋化因子及其受体相关基因表达的影响
- Author:
Shanshan HU
;
Hong YANG
;
Jian WANG
;
Yao YAO
;
Yongjian ZHU
;
Jiaming QIAN
- Keywords:
Pancreatic neoplasms;
ARHI gene;
Chemotactic factors;
Receptors;
chemokine
- From:
Chinese Journal of Pancreatology
2017;17(3):176-179
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of ARHI transfection on the chemokines and receptors related gene expression profile of PANC1 cells.Methods Plasmids expressing ARHI and empty plasmid were transfected into PANC1 cells, and the stably expressed cell lines were established by using G418.mRNA expression of chemokines and receptors related genes was detected by PCR Array.Real-time PCR was used to detect mRNA expression of the genes related vascular growth.Results In cells transfected with ARHI gene, the expression levels of mRNA of 36 genes were down-regulated, and 9 were up-regulated.Among the genes related to tumor metastasis and invasion CXCL12 and CXCR4 were significantly down-regulated (<-6 folds), and MMP-2 was slightly down-regulated (<-2 folds).Among the genes related to tumor angiogenesis, pro-angiogenesis genes including CCL2, CXCL1, CXCL2, CXCL8, CXCL12 and CXCR4 were significantly down-regulated, and pro-angiogenesis genes including CXCL3, CCR1 and CCR2 was slightly down-regulated.Anti-angiogenesis genes including CXCL9, CXCL10, CXCL11 and CXCR3 were significantly up-regulated (>6 folds).Among the genes related to the localization of distant organ infiltration and latency, CXCL12, CXCR4 and CCR7 were significantly down-regulated,and CXCR5 was slightly down-regulated.Among the gene with tumor immunity,CXCL8,CXCR1 and CCR7 were significantly down-regulated.Gene expression of CXCL1,CXCL8,CXCR4 and CXCR3 detected by Real-time PCR were consistent with PCR array.Conclusions ARHI gene inhibits the expression of chemokines and receptors related to tumor metastasis,angiogenesis and tumor immunity.
