Expression and clinical significance of FoxO1 in idiopathic congenital talipes equinovarus in Uigur of Xinjiang
10.3969/j.issn.1671-8348.2017.17.011
- VernacularTitle:FoxO1在新疆维吾尔族先天性马蹄内翻足的表达及其临床意义
- Author:
Guojie CHENG
;
Abudurexiti AIKEBAER
;
Jing WANG
;
Faming LV
;
Haojie DONG
- Keywords:
ICTEV;
FoxO1;
gene expression;
Uigur nationality;
degree of deformity
- From:
Chongqing Medicine
2017;46(17):2340-2342
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of FoxO1 gene in idiopathic congenital talipes equinovarus(ICTEV) in Xinjiang Uigur and its correlastion with deformity degree of ICTEV.Methods The FoxO1 protein expression level of foot muscle tissues in 51 Uigur children cases of ICTEV and 25 Uigur normal children were detected by Western blot,the relative expression level of FoxO1 mRNA in different deformity degrees of ICTEV was detected by using real time fluorescence quantitative PCR.Results The Western Blot detection results showed that the expression level of FoxO1 protein in ICTEV children patients was (0.52 ± 0.03),which was lower than(1.61 ± 0.15) in normal children,the difference was statistically significant (P =0.017).The real time fluorescence quantitative PCR detection showed that the relative expression level of FoxO1 mRNA in the Dimeglio type Ⅱ,Ⅲ and Ⅳ were 1.02±0.24,0.67±0.15 and 0.24±0.19 respectively.There was negative correlation between the deformity degree of ICTEV and FoxO1 mRNA relative expression level.Conclusion The transcription level of FoxO1 gene is gradually decreased with the deformity degree aggravation of ICTEV,suggesting that the FoxO1 gene may involve in the whole onset process of Uigur ICTEV.
