Determination of Entrapment Efficiency of Levodopa-loaded PEGylated-Solid Lipid Nanoparticles by Mini-Column Centrifugation-HPLC
10.3870/j.issn.1004-0781.2017.07.019
- VernacularTitle:微柱离心-HPLC法测定载左旋多巴聚乙二醇化固体脂质纳米粒的包封率
- Author:
Man WEI
;
Yujia ZHANG
;
Yuyuan CHEN
;
Nan ZHANG
;
Wensheng ZHENG
- Keywords:
Levodopa;
Solid lipid nanoparticles;
Microcolumn centrifugation;
Chromatography,high performance liquid;
Entrapment efficiency
- From:
Herald of Medicine
2017;36(7):797-800
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a mini-column centrifugation-HPLC method to determine the entrapment efficiency of levodopa-loaded PEGylated-solid lipid nanoparticles.Methods A dextran gel(Sephadex G-50) mini-column centrifugation was employed to separate the free drug from solid lipid nanoparticles.The content of levodopa was qualified by HPLC.Results Under the applied chromatographic condition,the excipients had no influence on the determination of levodopa.A calibrated linear of levodopa concentration was within 10.54-527.00 μg·mL-1.The recoveries of high,medium and low concentrations of levodopa were 99.13%,99.51% and 99.04%(RSD were 1.25%,1.91% and 1.71%), respectively.The free levodopa was well separated from solid lipid nanoparticles by using mini-column centrifugation.The addition of blank solid lipid nanoparticles recovery was 98.84% with RSD of 0.80%(n=3).The average adsorption rates of the three concentrations of free levodopa were 100.00%,98.75% and 98.56%(RSD were 0.00%,0.19% and 0.18%,n=3),respectively.The adsorption rate of the physical mixtures of three different concentrations of drugs and empty PEGylated solid lipid nanoparticles were 99.68%,98.46% and 99.21%(RSD were 1.52%,0.23% and 0.21%),respectively.Conclusion The method was simple,accurate and reproducible,which can be used for determination of the entrapment efficiency of levodopa-loaded PEGylated-solid lipid nanoparticles.
