Development and validation for the determination of total flavonoids and luteolin from Pteris multifida Poir
10.3760/cma.j.issn.1673-4246.2017.07.010
- VernacularTitle:建立凤尾草中总黄酮和木犀草素的含量测定方法
- Author:
Mengwei LI
;
Yuemei MA
;
Aoxue LIU
;
Hao WANG
;
Xinjing CHEN
;
Yang LIU
- Keywords:
Pteris multifida;
Flavones;
Chromatography,high pressure liquid;
Luteolin
- From:
International Journal of Traditional Chinese Medicine
2017;39(7):617-621
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the content of total flavonoids and luteolin from Pteris multifida Poir. Methods The content of total flavonoids was determined by gradient elution of macroporous resin D101 and ultraviolet spectrophotometry. The content of luteolin was determined by HPLC. The analysis was performed on a RP-C18 column(4.6 mm×250 mm, 5 μm) with aceconitrile-0.2% phosphoric acid (35:65) as the mobile phase at a flow rate of 1.0 ml/min, and 30 ℃ temperature. Results The detection of wave length was set at 349 nm. The content of luteolin was 0.015%, 0.019%, 0.016%, and the content of total flavonoids was 0.015%, 0.019%, 0.016%, respectively. Conclusions The method is suitable for the determination of flavonoids componets from Pteris multifida Poir.
