Protective effect of astragalosides IV on retinal pigmentepithelium injury induced by methylglyoxal
10.3969/j.issn.1001-1978.2017.07.007
- VernacularTitle:黄芪甲苷对甲基乙二醛诱导的人视网膜色素上皮细胞损伤的保护作用研究
- Author:
Yunfeng ZHOU
;
Lin LI
;
Zhengyan GE
;
Lidong ZHOU
;
Yujie GUO
;
Long JIN
;
Ye REN
;
Yanlin LI
;
Lan SUN
;
Yang XU
- Keywords:
diabetic retinopathy;
astragaloside IV;
methylglyoxal;
retinal pigment epithelium;
antioxidation;
apoptosis
- From:
Chinese Pharmacological Bulletin
2017;33(7):915-921
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the protective effect of astragaloside IV (AS-Ⅳ) on human retinal pigment epithelium injury induced by methylglyoxal (MGO), and explore its molecular mechanism.Methods The injury of ARPE-19 cells was induced by MGO and the cell viability was measured by CCK-8 method.The morphology of cell nucleus was analyzed by Hoechst 33342 staining and the cell apoptosis was analyzed by flow cytometry to detect labbled Annexin V-FITC/PI.JC-1 staining and fluorescence probe DCFH-DA were employed to evaluate the change of mitochondrial membrane potential and reactive oxygen species (ROS).The levels of SOD, MDA, caspase-9 and caspase-3 were determined by respective kits.Western blot was used to analyse the expression of Bcl-2, Bax and PARP.Results AS-Ⅳ could significantly inhibit the decrease of cell viability induced by MGO, improve the morphology of cell nucleus, reduce the ARPE-19 cell apoptosis rate and the level of ROS and MDA, and increase the activity of SOD.Furthermore, AS-Ⅳ could enhance mitochondrial membrane potential, the ratio of Bcl-2/Bax and the expression of PARP, and inhibit the activation of caspase-9 and caspase-3.Conclusion AS-Ⅳ may protect ARPE-19 cells from the injury induced by MGO by increasing the antioxidant ability of ARPE-19 cells and inhibiting cell apoptosis.
