Effects of cucurbitacin Ⅱa on apoptosis of humanlung cancer cell lines NCI-H460 and A549 and its mechanism
10.3969/j.issn.1001-1978.2017.07.008
- VernacularTitle:雪胆素甲诱导非小细胞肺癌系NCI-H460和A549的凋亡效应及其作用机制初探
- Author:
Yulin CHEN
;
Qing XIAN
;
Cui XIAO
;
Yueling ZHONG
;
Xiaomei SU
;
Li XU
;
Qiaoli LUO
;
Peng CHENG
;
Tao WANG
;
Jin LIU
;
Tao ZHANG
;
Tai YANG
;
Qiang ZOU
;
Hua LI
- Keywords:
curcerbitacin Ⅱa;
non-small-cell lung cancer;
apoptosis;
Aurora A;
Cofilin;
STAT3
- From:
Chinese Pharmacological Bulletin
2017;33(7):922-927
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the apoptosis effect of cucurbitacin Ⅱa on non-small cell lung cancer cell lines NCI-H460 and A549 and its underlying mechanism.Methods Cell viability was assessed by CCK-8 assay.The apoptosis effect and cell cycle arrest were detected by Flow cytometry.Western blot was employed to detect the related protein.Results The proliferation of lung cancer cell lines NCI-H460 and A549 was inhibited by CuⅡa, which showed cytotoxic activity with IC50 values of 224.9 nmol·L-1 and 108.3 nmol·L-1 against NCI-H460 and A549 respectively.CuⅡa induced the cells apoptosis and cell cycle arrest at G2/M phase.The results of Western blot showed CuⅡa inhibited the phosphorylation of STAT3 and Cofilin in a dose-dependent manner.Further, CuⅡa inhibited the phosphorylation of Aurora A, in line with the important characteristics of anti-tumor effect of Aurora A kinase inhibitor with blocking cells in the G2/M phase.Conclusion CuⅡa has obvious anti-tumor effect against non-small cell lung cancer, which suggests its value as a lead compound for lung cell carcinoma.
