Role of matrix metalloproteinase-9 down-regulation in learningand memory dysfunction induced by propofol treatment in rats
10.3969/j.issn.1001-1978.2017.07.012
- VernacularTitle:基质金属蛋白酶-9表达下调在异丙酚麻醉诱发大鼠学习记忆障碍中的作用
- Author:
Hongyou WANG
;
Ya YU
;
Zongping YI
;
Ai YAN
;
Ping CHEN
- Keywords:
propofol;
matrix metalloproteinase;
brain-derived neurotrophic factor;
rats;
hippocampus;
apoptosis
- From:
Chinese Pharmacological Bulletin
2017;33(7):946-950
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the role of matrix metalloproteinase-9 down-regulation in the learning and memory dysfunction induced by propofol treatment in rats.Methods 7-day-old SD rats were randomly divided into three groups(n=18):control group(NS group) and repeated doses of propofol group(RP group) was intraperitoneally injected with normal saline and propofol respectively for consecutive seven days, single dose of propofol group(SP group) were intraperitoneally injected with normal saline first for consecutive six days, and then injected with propofol on 7th day.The blood gas and glucose levels were monitored of six rats randomly selected from each group.Morris water maze was conducted to test the learning and memory functions of the remaining rats.The expression of MMP-9, BDNF and caspase-3 was detected by Western blot, and the hippocampal neuron apoptosis was determinated by TUNEL staining.Results Compared with NS group and SP group, the escape latency in RP group was prolonged significantly, exploration time and the number of crossing the platform in RP group were markedly decreased(P<0.05).The expressions of MMP-9 and mBDNF in RP group declined, but the expression of proBDNF and the ratio of proBDNF/mBDNF in RP group were higher than those in NS group and SP group(P<0.05).Compared with NS group and SP group, the number of apoptotic neurons and the expression of cleaved-caspase-3 in RP group were increased significantly, but the expression of pro-Caspase3 in RP group was reduced(P<0.05).There was no difference between SP group and NS group regarding all the results(P>0.05).Conclusions Repeated exposure to propofol can lead to a decline in long-term learning and memory functions in neonatal rats, which may be related to the down-regulation of MMP-9 expression, proBDNF and mBDNF conversion disorder in hippocampus and the apoptosis of hippocampal neurons.However, single exposure to propofol has no significant effect.
