High-efficiency expression and purification of the soluble glycoprotein extracellular domain of Rabies virus strain CTN

VernacularTitle:狂犬病病毒CTN株糖蛋白膜外区可溶性高效表达及纯化
Author: Jia LI ; Yafei LIU ; Yunpeng WANG ; Shouchun CAO ; Leitai SHI ; Yongxin YU ; Yuhua LI
Keywords: Rabies virus; Glycoprotein extracellular domain; Soluble protein expression
From: Chinese Journal of Microbiology and Immunology 2017;37(6):438-442
CountryChina
Language:Chinese
Abstract: Objective To express and purify the glycoprotein extracellular domain (Ex-GP) of Rabies virus strain CTN in soluble form with high efficiency.Methods A recombinant expression plasmid containing the gene encoding the Ex-GP was constructed.Various expression conditions were screened to obtain an optimum prokaryotic expression system for Ex-GP in soluble form.The expressed target protein was purified using affinity chromatography and gel filtration chromatography.Results The target protein Ex-GP with high antigenicity was efficiently expressed in soluble form by using the recombinant PBCX expression system and effectively purified by using affinity and gel filtration chromatography.Conclusion The soluble form of Ex-GP is successfully expressed and purified in a simple and convenient way.This study paves the way for further researches on the biological functions of rabies virus glycoprotein,the pathogenic mechanism of rabies and the development of diagnostic reagent and vaccines for rabies virus.