Expression level of miR-16 in Staphylococcus aureus sepsis and its clinical significance
10.3969/j.issn.1002-2694.2017.05.008
- VernacularTitle:miR-16在金黄色葡萄球菌脓毒症中的表达及意义探讨
- Author:
Jiaxue WANG
;
Yan CHEN
;
Xiaoxiang LIU
;
Aihua SUN
;
Jun YANG
;
Huoyang LYU
;
Yonglie ZHOU
- Keywords:
miR-16;
Staphylococcus aureus;
sepsis
- From:
Chinese Journal of Zoonoses
2017;33(5):427-431
- CountryChina
- Language:Chinese
-
Abstract:
We investigated correlation between the level of miR-16 expression and the severity of Staphylococcus aureus sepsis,and further explored its potentially clinical significance.Blood samples were collected from 32 patients,including each 8 cases of septic shock,severe sepsis and general sepsis,as well as 8 cases of healthy volunteers.Blood samples from 24 cases of healthy subjects with different ages were measured,and additionally 8 cases of blood samples from gram negative bacteria sepsis were also determined in current study as a control.Trizol solution for the whole blood lysis was added into blood samples,and followed by the extraction of microRNA.The expression levels of miR-16 in different groups were measured by fluorescence quantitative PCR,in which 2-Delta Ct method was used.SPSS software (version 13.0) was used to analyze the statistical differences between the groups,and further analyze the correlation between miR-16 value and the corresponding CRP and PCT values.Results showed that the expression level of miR-16 was negatively correlated with the severity of Staphylococcus aureus sepsis.There were statistically significant differences in experimental groups when compared with the control (P＜0.001),and there was also a statistically significant difference between each experimental group (P＜0.01).We found that the expression level of miR-16 was negatively correlated with CRP and PCT,the correlation coefficients were-0.561 and-0.769 respectively,and trend analysis showed that there was a significantly negative correlation.A significantly negative correlation was found between the miR-16 expression level and severity of sepsis,suggesting that miR-16 may serve as a biomarker for the severity of Staphylococcus aureus sepsis.
