Genotyping of Yersinia pestis isolated from Gansu Province by clustered regularly interspaced short palindromic repeats
10.3760/cma.j.issn.2095-4255.2017.06.004
- VernacularTitle:规律成簇的间隔短回文重复序列对甘肃省鼠疫菌株的基因分型研究
- Author:
Yajun GE
;
Pengfei GE
;
Jinxiao XI
;
Wei LI
;
Hong ZHANG
;
Na MA
;
Bin WU
;
Limin GUO
;
Daqin XU
- Keywords:
Yersinia pestis;
Ecotype;
Genotyping
- From:
Chinese Journal of Endemiology
2017;36(6):404-407
- CountryChina
- Language:Chinese
-
Abstract:
Objective To type Yersinia pestis isolated from Gansu Province,and to study the trend of diffreent strains in different administrative regions and different years.Methods Totally 193 strains were enrolled in this study,including 9 strains of Ganning Dauricus type,18 strains of Aerjin type,45 strains of Qilian type and 121 strains of Qingzang type.These strains were genotyped by clustered regularly interspaced short palindromic repeats (CRISPR),and genotypes were named according to international standard.Genotyping by CRISPR in different administrative regions and different years of Gansu Province was explored.Results Two clusters (Ca7 and Cb4),including four genotypes (genotypes 7,22,24 and 26) were classified by CRISPR.From the point of view of origin,genotype 24 was the main genotype in Akesai 36.36％ (16/44),Subei 36.17％ (17/47),Yumen 50.00％ (5/10) and Su'nan 38.67％ (29/75);the main genotype of Xiahe and Huining was genotype 26 (4/7);the main genotype of Shandan was genotype 22 (1/1).From the point of view of time,the main genotype of Yersiniapestis in Gansu Province during the years of 1960-1969,1970-1979 and 1980-1989 was genotype 26 [53.33％ (8/15),60.00％ (6/10) and 48.28％ (14/29)];the main genotype was genotype 22 [40.91％ (18/44)] during the years of 1990-1999;and the main genotype was genotype 24 [43.16％ (41/95)] during the years of 2000-2009.Conclusion Four genotypes of Yersiniapestis in Gansu Province are quite different in different administrative regions and different years.
