Expression and clinical significance of circulating miR-103a-2 in serum from patients with nonalcoholic fatty liver disease
10.13602/j.cnki.jcls.2017.05.07
- VernacularTitle:血清循环miR-103a-2在非酒精性脂肪性肝病中的表达及意义
- Author:
Xu WANG
;
Danni LI
;
Yuxiao FU
;
Hui FENG
- Keywords:
nonalcoholic fatty liver disease;
hyperlipemia;
miRNA;
miR-103a;
fluorescent real-time quantitative PCR
- From:
Chinese Journal of Clinical Laboratory Science
2017;35(5):346-348
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the significance of miR-103 as noninvasive biomarker for diagnosis of nonalcoholic fatty liver disease (NAFLD).Methods The serum samples were collected from healthy subjects and NALFD patients to detect biochemical parameters.NucleoZOL method was used to isolate serum miRNA.SYBR Green qPCR was used for relatively quantitative analysis of miR103a-2.The correlations between miR-103a-2 and biochemical parameters were analyzed by Spearman method.Results Compared with healthy control group,the levels of total cholesterol (TC),triacylglyceride (TG),alanine aminotransferase (ALT) and glutamyl transpeptidase (γ-GGT) were all significantly increased in NAFLD group (P ＜ 0.01).Compared with the NAFLD group accompanying ortholiposis,the levels of TC,TG and low density lipoprotein-cholesterol (LDL-C) were all significantly increased in the NAFLD group accompanying dyslipidemia (P ＜0.01).Compared with the healthy control group,the level of serum miR-103a-2 increased in both NAFLD with ortholiposis group (P ＜ 0.05) and NAFLD with dyslipidemia group (P ＜ 0.01).The level of serum miR-103a-2 was positively correlated with serum TC (r =0.495,P =0.001).Conclusion Serum miR-103a-2 may be a more sensitive parameter for noninvasive screening of NAFLD than the parameters of blood lipid.
