Metabolomics Research on Compound Danshen DrippingPills in Acute Myocardial Infarction Rat

VernacularTitle:复方丹参滴丸抗急性心肌梗死代谢组学研究
Author: Qinwei LU ; Ling TONG ; Dongxiang LI ; Fengguo XU
Keywords: Compound Danshen dripping pills; Acute myocardial infarction; Metabolomics; Plasma
From: Chinese Journal of Analytical Chemistry 2017;45(6):791-798
CountryChina
Language:Chinese
Abstract: An acute myocardial infarction rat model was established by ligation of the left ventricular coronary artery.Plasma samples of rats were collected and analyzed by ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS) to study the myocardial protection mechanism of compound Danshen dropping pill (CDDP).After principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA), 22 metabolites were identified as potential biomarker of AMI.Furthermore, CDDP had remarkable effect on AMI rats.p-Tolyl sulfate, hippuric acid, equol 7-O-glucuronide, lysoPC(16∶0), cholic acid, oleamide, palmitic amide and SM(d18∶1/16∶0) were significantly changed in treatment group.The results showed that CDDP had a very good myocardial protection effect on AMI rats, and might influence the pathways of phenylalanine metabolism, glycerophospholipid metabolism, fatty acid metabolism, primary bile acid biosynthesis and Sphingolipid metabolism.