Combination of High Performance Liquid Chromatographywith Nano-electrospray ionization Chirp Z TransformIon Mobility Spectrometry
10.11895/j.issn.0253-3820.160859
- VernacularTitle:高效液相色谱与纳流电喷雾阵列Chirp Z变换离子迁移谱联用方法研究
- Author:
Hongling SHEN
;
Qingyan MENG
;
Xu JIA
;
Wenjie LIU
- Keywords:
Chirp Z transform;
Ion mobility spectrometry;
High performance liquid chromatography
- From:
Chinese Journal of Analytical Chemistry
2017;45(6):809-816
- CountryChina
- Language:Chinese
-
Abstract:
By combining frequency modulation Chirp Z transform ion mobility spectrometers (IMS) and multi nozzle electrospray array ionization source, a method of NanoESI-Chirp Z transform ion mobility spectrometry-high performance liquid chromatography was developed for the determination of n-alkyl ammonium bromide compounds.The parameters of NanoESI-Chirp Z transform IMS such as electric field intensity, solvent composition, and solution flow rate were investigated and optimized.Subsequently, four kinds of n-alkyl ammonium bromide compounds were respectively detected by this developed Chirp Z transform method and Fourier transform method, and the obtained results were compared.The result indicated that the optimum conditions were electric field intensity of 4.5 kV, and ESI solution flow rate of 8 μL/min.Then a test mixture containing tetrabutylammonium bromide, tetrapentylammoniumbromide, tetrahexylammonium bromide, tetraheptylammonium bromide, tetranoctylammoniumbromideandtetrakis(decyl) ammonium bromide was successfully separated and determined by the HPLC-nanoESI-Chirp Z IMS method.Chirp Z transform method provided higher signal to noise ratio compared to conventional signal averaging method, and was superior to FT method in the determination of drift time.
