Effects of Psoralen on Alcohol-induced Proliferation of Osteoblasts
10.13359/j.cnki.gzxbtcm.2017.04.020
- VernacularTitle:补骨脂素对酒精诱导的成骨细胞增殖的影响
- Author:
Liwei HUO
;
Guangwei WANG
;
Hong HUANG
;
Hongxing HUANG
;
Weizhong YU
;
Lei WAN
- Keywords:
psoralen/pharmacology;
alcohol-induced osteoporosis;
osteoblasts;
cell proliferation;
cell culture
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2017;34(4):555-559
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of psoralen on the proliferation of osteoblasts induced by alcohol, thus to explore the mechanism of psoralen in preventing and treating alcohol-induced osteoporosis. Methods The osteoblasts separated from neonatal rat skull were identified by alkaline phosphatase (ALP) staining method. The in-vitro confirmed osteoblasts were randomly divided into 4 groups, namely blank control group, alcohol group (model group), psoralen group, and psoralen plus alcohol group. The alcohol-induced osteoblast proliferation in various groups was determined by methyl thiazolyl tetrazolium (MTT) method. Results Compared with the blank control group, the alcohol-induced osteoblast proliferation at culturing hour 24, 48, 72, 96 in alcoholgroup was significantly decreased (P<0.05), and the proliferation in psoralen group at various time points only showed an increasing trend, the differences being statistically insignificant (P>0.05). Compared with the alcohol group, osteoblast proliferation at culturing hour 24 in psoralen plus alcohol group was significantly increased (P <0.05) , but the differences of osteoblast proliferation at culturing hour 48, 72, 96 h between the two groups were insignificant (P > 0.05). Conclusion Psoralen has certain effect on promoting alcohol-induced osteoblast proliferation in vitro.
